实验技术服务

完善的实验室及设备

赛哲生物拥有近1000㎡的中心实验室。
配置有公共实验室、PCR实验室、产物分析室、细胞实验室、样本处理实验室、建库实验室、测序室、病毒实验室等等。

我们拥有Illumina系列的多台高通量测序仪、LGC oKtopure全自动核酸抽提工作平台、Hydrocycler2全自动水浴PCR工作平台、IntelliQube全自动一体化实时荧光定量PCR工作平台、NanoSight NS300纳米颗粒跟踪分析仪、ProteinSimple Wes全自动蛋白质印迹定量分析系统、BECKMAN COULTER 超速离心机、BD 流式细胞仪、ABI 定量 PCR 仪、Qiagen QIAcube 全自动核酸纯化仪、安捷伦/Agilent 2100 芯片生物分析仪等各种高端实验仪器。

我们的技术实力

赛哲生物现已建立由多名科技特派员、科研院所教授组成的科学家顾问团队,
以及由多名海外留学博士带领、具备多学科交叉互补能力的高素质研发服务团队,
致力于为客户提供高通量测序、实验服务、科研试剂和临床诊断领域的相关配套服务。

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7年科研服务经验

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合作文章影响因子7000+

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丰富的样本类型提取经验及项目经验

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科研大咖技术团队

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从项目设计到文章插图一站式服务

多领域的实验技术服务,解决多种实验难题

赛哲生物是国内首家提供3D细胞培养服务的公司。

我们提供基因组学、细胞生物学、蛋白质组学、病理检测、病毒包装、动物实验等整体项目技术服务,涵盖:蛋白分子互作机理研究、蛋白纯化、蛋白质修饰检测、细胞流式、DNA甲基化检测等分子生物学、细胞生物学、蛋白质组学和代谢组学等诸多领域。

  • 业务咨询热线:400-888-4242(转1)
  • 科服业务邮箱:sagene_sales@sagene.com.cn

全方位解决方案

分子克隆服务

  • 荧光定量PCR(qPCR检测)
  • 基因调取及载体构建
  • RACE扩增
  • 甲基化检测
  • SNP分型
  • 3’UTR活性检测
  • 启动子活性检测
  • 菌种鉴定
  • 基因扩增和测序
  • 质粒提取

蛋白及互作相关服务

  • 全自动WB检测
  • ELISA检测
  • RNA interactome实验
  • RNA pull down实验
  • DNA pull down实验
  • ChIP实验
  • RIP实验
  • CoIP实验
  • 蛋白原核表达
  • 标签蛋白pull down实验
  • LC-MS/MS质谱鉴定

细胞功能服务

  • 细胞培养及处理
  • 3D血管生成
  • 血液中PBMC分离
  • 流式细胞检测
  • 细胞增殖能力检测(MTT/CCK-8)
  • Transwell细胞侵袭实验
  • siRNA筛选
  • 稳定细胞株构建
  • 免疫组化
  • 免疫荧光
  • 电镜拍照(透射/扫描电镜)
  • 激光共焦实验

组学服务

  • Labelfree
  • iTRAQ
  • MRM
  • SWATH/PRM

外泌体检测项目

  • 外泌体抽提
  • 外泌体WB检测
  • 外泌体NTA检测
  • 外泌体电镜检测

部分合作单位及发表文章

Mai Z, Xiao C, Jin J, et al. Low-cost, Low-bias and Low-input RNA-seq with High Experimental Verifiability based on Semiconductor Sequencing[J]. Scientific Reports, 2017, 7(1): 1053.[IF 5.228]
Dai C, Shen J, Ren Y, et al. Choice of surgical procedure for patients with non–small-cell lung cancer≤ 1 cm or> 1 to 2 cm among lobectomy, segmentectomy, and wedge resection: A population-based study[J]. Journal of Clinical Oncology, 2016, 34(26): 3175-3182.[IF 20.983]
Sun T T, He J, Liang Q, et al. LncRNA GClnc1 Promotes Gastric Carcinogenesis and May Act as a Modular Scaffold of WDR5 and KAT2A Complexes to Specify the Histone Modification Pattern[J]. Cancer discovery, 2016, 6(7): 784-801.[IF 19.78]
Zheng W, Yin T, Chen Q, et al. Co-delivery of Se nanoparticles and pooled SiRNAs for overcoming drug resistance mediated by P-glycoprotein and class III β-tubulin in drug-resistant breast cancers[J]. Acta biomaterialia, 2016, 31: 197-210.[IF 6.008]
Chen Z, Chen Y, Xu M, et al. Osimertinib (AZD9291) Enhanced the Efficacy of Chemotherapeutic Agents in ABCB1-and ABCG2-Overexpressing Cells In Vitro, In Vivo, and Ex Vivo[J]. Molecular cancer therapeutics, 2016.[IF 5.58]
Chen W, Li S, Li J, et al. Artemisitene activates the Nrf2-dependent antioxidant response and protects against bleomycin-induced lung injury[J]. The FASEB Journal, 2016, 30(7): 2500-2510.[IF 5.30]
Liu J E, Ren B, Tang L, et al. The independent contribution of miRNAs to the missing heritability in CYP3A4/5 functionality and the metabolism of atorvastatin[J]. Scientific reports, 2016, 6.[IF 5.228]
Dong P, Xiong Y, Watari H, et al. Suppression of iASPP-dependent aggressiveness in cervical cancer through reversal of methylation silencing of microRNA-124[J]. Scientific reports, 2016, 6.[IF 5.228]
Zhang Y, Su H, Wen L, et al. Mathematical modeling for local trans-round window membrane drug transport in the inner ear[J]. Drug delivery, 2016, 23(8): 3082-3087.[IF 4.843]
Wang J, Chen H, Zhou Y, et al. Atorvastatin inhibits myocardial apoptosis in a swine model of coronary microembolization by regulating PTEN/PI3K/Akt signaling pathway[J]. Cellular Physiology and Biochemistry, 2016, 38(1): 207-219.[IF 4.652]
Fu F, Chen F, Li R, et al. Prenatal diagnosis of fetal multicystic dysplastic kidney via high-resolution whole-genome array[J]. Nephrology Dialysis Transplantation, 2016: gfv465.[IF 4.085]
Yin Y, Xu Y, Su L, et al. Epidemiologic investigation of a family cluster of imported ZIKV cases in Guangdong, China: probable human-to-human transmission[J]. Emerging Microbes & Infections, 2016, 5(9): e100.[IF 4.012]
Yu X, Liu L, Chen W, et al. Integrity of Amygdala Subregion-Based Functional Networks and Emotional Lability in Drug-Naïve Boys With ADHD[J]. Journal of Attention Disorders, 2016: 1087054716661419.[IF 3.384]
Zheng Z, Liang J, Huang R, et al. Identification of a novel miR-146a from Pinctada martensii involved in the regulation of the inflammatory response[J]. Fish & shellfish immunology, 2016, 54: 40-45.[IF 3.025]
Liang Y X, Lu J M, Mo R J, et al. E2F1 promotes tumor cell invasion and migration through regulating CD147 in prostate cancer[J]. International journal of oncology, 2016, 48(4): 1650-1658.[IF 3.018]
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